Hitrap q ff manual

AS 5 Table 2. HiTrap SP HP and HiTrap Q HP columns characteristics 1 Median particle size of the cumulative volume distribution. 2 Dynamic binding capacity measured at 1 mL/min (1 min residence time) using M sodium acetate, pH 3 Dynamic binding capacity at 10% breakthrough by frontal analysis at a mobile phase velocity of cm/h in a PEEK /50 . Q Sepharose Fast Flow columns Ease of scale-up is a key benefit of working with any Sepharose Fast Flow ion exchanger. In Figure 3, a purification is scaled up first five-fold and then twenty-fold on prepacked HiTrap Q FF and HiPrep Q FF 16/10 columns, respectively. Column: HiPrep Q FF 16/10 Sample: E. coli lysate with expressed GFP-(histidine) 6. HiTrap ® Q FF is prepacked with Q Sepharose ® Fast Flow, and is a strong anion exchanger for small-scale protein purifications as well as screening of binding and elutions conditions. Q, SP, DEAE, and CM Sepharose ® Fast Flow are based on a robust, 6% highly cross-linked beaded agarose matrix with good flow properties and high loading www.doorway.ru: CYTIVA.

Q: approx. 50 mg HSA/ml medium (20 mM Tris-HCl, pH at 1 ml/min) Mean particle size 34 µm Bead structure 6% highly cross-linked spherical agarose Maximum flow rates1 HiTrap 1 ml: 4 ml/min, HiTrap 5 ml: 20 ml/min Recommended flow rates HiTrap 1 ml: 1 ml/min, HiTrap 5 ml: 5 ml/min Chemical stability All commonly used buffers. 4 AK Table 1. Characteristics of HiTrap DEAE FF, CM FF, Q FF, SP FF and ANX FF columns. 1 At room temperature in water. Note: The pressure over the packed bed varies depending on a. Q Sepharose Fast Flow columns Ease of scale-up is a key benefit of working with any Sepharose Fast Flow ion exchanger. In Figure 3, a purification is scaled up first five-fold and then twenty-fold on prepacked HiTrap Q FF and HiPrep Q FF 16/10 columns, respectively. Column: HiPrep Q FF 16/10 Sample: E. coli lysate with expressed GFP-(histidine) 6.

HiTrap Q FF, 1 ml. HiTrap Q FF, 5 ml. RESOURCE Q, 1 ml. RESOURCE Q, 6 ml. Procedure: Use the flow rate recommended in the column instructions. Handbook. Ion Exchange Chromatography. Principles and Methods. use 1 ml and 5 ml columns, HiTrap Q and HiTrap SP respectively. Manual desalting with HiTrap columns. HiTrap rProtein A FF for the capture step. The flow rate normalized by the column cross section (cm/h).